Biology Chapter 23 Biotechnology Mcqs Test Online Punjab Boards 12th Class

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Question 1 of 15

Biology

1. Which technique rapidly replicates specific DNA fragments without cloning?

1.Gel electrophoresis

2.polymerase chain reaction

3.cDNA libraries

4.genetic probe

Question 1 of 15

Question 2 of 15

2. Which of the following is highly polymorphic molecular marker which is useful in DNA fingerprinting?

1.Palindromic DNA sequence

2.A short tandeam repeats

3.Cloned DNA sequence

4.Cosmid cloning vector

Question 2 of 15

Question 3 of 15

3. DNA molecules with complementary sticky ends associate by:

1.Disulphide bond

2.Ionic bond

3.Hydrogen bond

4.Covalent bond

Question 3 of 15

Question 4 of 15

4. A paleontologist, has recovered a bit of tissue from the 400 year old preserved skin of an extinct bird. The researcher would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of the bird DNA available for testing?

1.a RFLP analysis

2.polymerase chain reaction (PCR)

3.southern hybridization

4.electroporation

Question 4 of 15

Question 5 of 15

5. A Plasmid:

1.Is a type of bacteriophage

2.Is used as DNA vector

3.Is a retrovirus

4.Is a type of cDNA

Question 5 of 15

Question 6 of 15

6. A Genomic DNA library:

1.Represents all the DNA in a specific chromosome

2.Is made using reverse transcriptase

3.Is stored in a collection of recombinant bacteria

4.Is a DNA copy of mature mRNAs.

Question 6 of 15

Question 7 of 15

7. Gel electrophoresis separates nucleic acid on the basis of difference:

1.Length (molecular weight)

2.Relative proportion of adenine and guanine

3.Nucleotide sequence

4.Charge

Question 7 of 15

Question 8 of 15

8. The PCR technique polymerase chain reaction:

1.DNA ligase

2.Reverse transcriptase

3.Heat resistance DNA polymerase

4.Restriction enzymes

Question 8 of 15

Question 9 of 15

9. A cDNA clone contains:

1.Exons

2.mRNA

3.Anticodon

4.Introns

Question 9 of 15

Question 10 of 15

10. If eukaryotic DNA contains five cleavage sites for a particular restriction enzyme, how many fragments will be produced upon complete digestion of the DNA with that enzyme:

1.2

2.None

3.6

4.4

Question 10 of 15

Question 11 of 15

11. Plants are more readily manipulated by genetic engineering than are animals because:

1.A somatic plant cell can often give rise to a complete plant

2.Plants genes do not contain interon.

3.Plant cell have large nuclei

4.Genes can be inserted into plant cells by microinjection

Question 11 of 15

Question 12 of 15

12. The Dideoxynucleotides (ddATP, ddTTP, ddGTP, and ddCTP) are improvement in DNA sequencing because they:

1.Are not affected by high temperature

2.Are used as prime

3.Cause premature termination of growing DNA strand

4.Cause the DNA fragments that contain them to migrate more slowly through the gel

Question 12 of 15

Question 13 of 15

13. Which of the following sequences in double-stranded DNA is mostly likely to be recognized as a cutting site for a restriction enzyme?

1.GGCC CCGG

2.AAGG TTCC

3.AC CA TGGT

4.AGTC TCAG

Question 13 of 15

Question 14 of 15

14. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

1.DNA Ligase ----- enzyme that cuts DNA, creating the sticky ends of restriction fragments

2.Electrophoresis ------ separation of DNA fragments

3.Reverse transcriptase ------ production of cDNA from mRNA.

4.Restriction enzyme ------ production of RFLPs.

Question 14 of 15

Question 15 of 15

15. The Ti plasmid carried by Agrobacterium tumefaciens, is especially useful for introducing genes into a:

1.Animals

2.Yeast

3.Plant

4.Bacteria

Question 15 of 15

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