Biology Chapter 23 Biotechnology Mcqs Test Online Punjab Boards 12th Class

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Question 1 of 15

Biology

1. Which technique rapidly replicates specific DNA fragments without cloning?

1.polymerase chain reaction

2.genetic probe

3.cDNA libraries

4.Gel electrophoresis

Question 1 of 15

Question 2 of 15

2. A paleontologist, has recovered a bit of tissue from the 400 year old preserved skin of an extinct bird. The researcher would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of the bird DNA available for testing?

1.a RFLP analysis

2.electroporation

3.southern hybridization

4.polymerase chain reaction (PCR)

Question 2 of 15

Question 3 of 15

3. The Dideoxynucleotides (ddATP, ddTTP, ddGTP, and ddCTP) are improvement in DNA sequencing because they:

1.Cause the DNA fragments that contain them to migrate more slowly through the gel

2.Are not affected by high temperature

3.Are used as prime

4.Cause premature termination of growing DNA strand

Question 3 of 15

Question 4 of 15

4. If eukaryotic DNA contains five cleavage sites for a particular restriction enzyme, how many fragments will be produced upon complete digestion of the DNA with that enzyme:

1.2

2.6

3.None

4.4

Question 4 of 15

Question 5 of 15

5. Which of the following is highly polymorphic molecular marker which is useful in DNA fingerprinting?

1.Cloned DNA sequence

2.A short tandeam repeats

3.Palindromic DNA sequence

4.Cosmid cloning vector

Question 5 of 15

Question 6 of 15

6. A Genomic DNA library:

1.Represents all the DNA in a specific chromosome

2.Is a DNA copy of mature mRNAs.

3.Is stored in a collection of recombinant bacteria

4.Is made using reverse transcriptase

Question 6 of 15

Question 7 of 15

7. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

1.Electrophoresis ------ separation of DNA fragments

2.DNA Ligase ----- enzyme that cuts DNA, creating the sticky ends of restriction fragments

3.Restriction enzyme ------ production of RFLPs.

4.Reverse transcriptase ------ production of cDNA from mRNA.

Question 7 of 15

Question 8 of 15

8. Which of the following sequences in double-stranded DNA is mostly likely to be recognized as a cutting site for a restriction enzyme?

1.AAGG TTCC

2.GGCC CCGG

3.AGTC TCAG

4.AC CA TGGT

Question 8 of 15

Question 9 of 15

9. Gel electrophoresis separates nucleic acid on the basis of difference:

1.Nucleotide sequence

2.Length (molecular weight)

3.Charge

4.Relative proportion of adenine and guanine

Question 9 of 15

Question 10 of 15

10. A Plasmid:

1.Is a retrovirus

2.Is a type of cDNA

3.Is used as DNA vector

4.Is a type of bacteriophage

Question 10 of 15

Question 11 of 15

11. DNA molecules with complementary sticky ends associate by:

1.Covalent bond

2.Disulphide bond

3.Hydrogen bond

4.Ionic bond

Question 11 of 15

Question 12 of 15

12. The PCR technique polymerase chain reaction:

1.Restriction enzymes

2.Reverse transcriptase

3.DNA ligase

4.Heat resistance DNA polymerase

Question 12 of 15

Question 13 of 15

13. The Ti plasmid carried by Agrobacterium tumefaciens, is especially useful for introducing genes into a:

1.Animals

2.Yeast

3.Plant

4.Bacteria

Question 13 of 15

Question 14 of 15

14. A cDNA clone contains:

1.Introns

2.Anticodon

3.Exons

4.mRNA

Question 14 of 15

Question 15 of 15

15. Plants are more readily manipulated by genetic engineering than are animals because:

1.Plants genes do not contain interon.

2.A somatic plant cell can often give rise to a complete plant

3.Genes can be inserted into plant cells by microinjection

4.Plant cell have large nuclei

Question 15 of 15

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