Biology Chapter 23 Biotechnology Mcqs Test Online Punjab Boards 12th Class

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Question 1 of 15

Biology

1. DNA molecules with complementary sticky ends associate by:

1.Disulphide bond

2.Covalent bond

3.Ionic bond

4.Hydrogen bond

Question 1 of 15

Question 2 of 15

2. The PCR technique polymerase chain reaction:

1.Heat resistance DNA polymerase

2.Reverse transcriptase

3.Restriction enzymes

4.DNA ligase

Question 2 of 15

Question 3 of 15

3. Which of the following sequences in double-stranded DNA is mostly likely to be recognized as a cutting site for a restriction enzyme?

1.AGTC TCAG

2.AC CA TGGT

3.AAGG TTCC

4.GGCC CCGG

Question 3 of 15

Question 4 of 15

4. Which of the following is highly polymorphic molecular marker which is useful in DNA fingerprinting?

1.A short tandeam repeats

2.Palindromic DNA sequence

3.Cosmid cloning vector

4.Cloned DNA sequence

Question 4 of 15

Question 5 of 15

5. Gel electrophoresis separates nucleic acid on the basis of difference:

1.Nucleotide sequence

2.Relative proportion of adenine and guanine

3.Length (molecular weight)

4.Charge

Question 5 of 15

Question 6 of 15

6. The Ti plasmid carried by Agrobacterium tumefaciens, is especially useful for introducing genes into a:

1.Plant

2.Bacteria

3.Animals

4.Yeast

Question 6 of 15

Question 7 of 15

7. The Dideoxynucleotides (ddATP, ddTTP, ddGTP, and ddCTP) are improvement in DNA sequencing because they:

1.Are not affected by high temperature

2.Are used as prime

3.Cause premature termination of growing DNA strand

4.Cause the DNA fragments that contain them to migrate more slowly through the gel

Question 7 of 15

Question 8 of 15

8. Which technique rapidly replicates specific DNA fragments without cloning?

1.cDNA libraries

2.genetic probe

3.polymerase chain reaction

4.Gel electrophoresis

Question 8 of 15

Question 9 of 15

9. Plants are more readily manipulated by genetic engineering than are animals because:

1.A somatic plant cell can often give rise to a complete plant

2.Plant cell have large nuclei

3.Plants genes do not contain interon.

4.Genes can be inserted into plant cells by microinjection

Question 9 of 15

Question 10 of 15

10. A Genomic DNA library:

1.Is made using reverse transcriptase

2.Represents all the DNA in a specific chromosome

3.Is stored in a collection of recombinant bacteria

4.Is a DNA copy of mature mRNAs.

Question 10 of 15

Question 11 of 15

11. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

1.Reverse transcriptase ------ production of cDNA from mRNA.

2.Electrophoresis ------ separation of DNA fragments

3.DNA Ligase ----- enzyme that cuts DNA, creating the sticky ends of restriction fragments

4.Restriction enzyme ------ production of RFLPs.

Question 11 of 15

Question 12 of 15

12. If eukaryotic DNA contains five cleavage sites for a particular restriction enzyme, how many fragments will be produced upon complete digestion of the DNA with that enzyme:

1.None

2.4

3.2

4.6

Question 12 of 15

Question 13 of 15

13. A paleontologist, has recovered a bit of tissue from the 400 year old preserved skin of an extinct bird. The researcher would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of the bird DNA available for testing?

1.polymerase chain reaction (PCR)

2.southern hybridization

3.electroporation

4.a RFLP analysis

Question 13 of 15

Question 14 of 15

14. A cDNA clone contains:

1.mRNA

2.Exons

3.Introns

4.Anticodon

Question 14 of 15

Question 15 of 15

15. A Plasmid:

1.Is a type of cDNA

2.Is a type of bacteriophage

3.Is a retrovirus

4.Is used as DNA vector

Question 15 of 15

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