Biology Chapter 23 Biotechnology Mcqs Test Online Punjab Boards 12th Class

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Question 1 of 15

Biology

1. Which of the following is highly polymorphic molecular marker which is useful in DNA fingerprinting?

1.Cosmid cloning vector

2.Palindromic DNA sequence

3.Cloned DNA sequence

4.A short tandeam repeats

Question 1 of 15

Question 2 of 15

2. Plants are more readily manipulated by genetic engineering than are animals because:

1.Plant cell have large nuclei

2.Genes can be inserted into plant cells by microinjection

3.Plants genes do not contain interon.

4.A somatic plant cell can often give rise to a complete plant

Question 2 of 15

Question 3 of 15

3. A paleontologist, has recovered a bit of tissue from the 400 year old preserved skin of an extinct bird. The researcher would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of the bird DNA available for testing?

1.polymerase chain reaction (PCR)

2.electroporation

3.southern hybridization

4.a RFLP analysis

Question 3 of 15

Question 4 of 15

4. A Plasmid:

1.Is used as DNA vector

2.Is a type of cDNA

3.Is a type of bacteriophage

4.Is a retrovirus

Question 4 of 15

Question 5 of 15

5. If eukaryotic DNA contains five cleavage sites for a particular restriction enzyme, how many fragments will be produced upon complete digestion of the DNA with that enzyme:

1.2

2.None

3.6

4.4

Question 5 of 15

Question 6 of 15

6. Gel electrophoresis separates nucleic acid on the basis of difference:

1.Nucleotide sequence

2.Relative proportion of adenine and guanine

3.Charge

4.Length (molecular weight)

Question 6 of 15

Question 7 of 15

7. The Dideoxynucleotides (ddATP, ddTTP, ddGTP, and ddCTP) are improvement in DNA sequencing because they:

1.Are used as prime

2.Cause the DNA fragments that contain them to migrate more slowly through the gel

3.Are not affected by high temperature

4.Cause premature termination of growing DNA strand

Question 7 of 15

Question 8 of 15

8. Which of the following sequences in double-stranded DNA is mostly likely to be recognized as a cutting site for a restriction enzyme?

1.GGCC CCGG

2.AC CA TGGT

3.AAGG TTCC

4.AGTC TCAG

Question 8 of 15

Question 9 of 15

9. The PCR technique polymerase chain reaction:

1.Reverse transcriptase

2.Heat resistance DNA polymerase

3.Restriction enzymes

4.DNA ligase

Question 9 of 15

Question 10 of 15

10. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

1.Reverse transcriptase ------ production of cDNA from mRNA.

2.Restriction enzyme ------ production of RFLPs.

3.DNA Ligase ----- enzyme that cuts DNA, creating the sticky ends of restriction fragments

4.Electrophoresis ------ separation of DNA fragments

Question 10 of 15

Question 11 of 15

11. A Genomic DNA library:

1.Represents all the DNA in a specific chromosome

2.Is a DNA copy of mature mRNAs.

3.Is stored in a collection of recombinant bacteria

4.Is made using reverse transcriptase

Question 11 of 15

Question 12 of 15

12. The Ti plasmid carried by Agrobacterium tumefaciens, is especially useful for introducing genes into a:

1.Bacteria

2.Yeast

3.Plant

4.Animals

Question 12 of 15

Question 13 of 15

13. A cDNA clone contains:

1.mRNA

2.Introns

3.Anticodon

4.Exons

Question 13 of 15

Question 14 of 15

14. Which technique rapidly replicates specific DNA fragments without cloning?

1.cDNA libraries

2.Gel electrophoresis

3.genetic probe

4.polymerase chain reaction

Question 14 of 15

Question 15 of 15

15. DNA molecules with complementary sticky ends associate by:

1.Disulphide bond

2.Hydrogen bond

3.Covalent bond

4.Ionic bond

Question 15 of 15

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